Hotspot

psbE-petL

LSC · 62691-63962 · 1272 bp

Back to Homolepis

Pi 0.000786

MarkerSeek score 48.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1272
variable_sites: 1
pi: 0.000786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAACGTTCTCCCGTG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1346 bp	1.0	70.9
psbE-petL_p2	CAAAAGAACGTTCTCCCGTG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	1344 bp	1.0	70.9
psbE-petL_p3	CAGCAAAAGAACGTTCTCCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1349 bp	1.0	70.7
psbE-petL_p4	CAGCAAAAGAACGTTCTCCC	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	1347 bp	1.0	70.7
psbE-petL_p5	AGCAAAAGAACGTTCTCCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.8	58.5	1348 bp	1.0	70.1