Hotspot

rbcL-psaI

LSC · 57197-57926 · 730 bp

Back to Homolepis

Pi 0.00411

MarkerSeek score 61.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 3
pi: 0.00411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	ATCAAATTTGAGTTCGCGCC	AAGGCACGAAAATTGAGGGT	45.0	45.0	57.7	58.0	809-1036 bp	1.0	78.1
rbcL-psaI_p2	CGCAGCTTGTGAAGTATGGA	AAGGCACGAAAATTGAGGGT	50.0	45.0	58.3	58.0	834-1061 bp	1.0	78.0
rbcL-psaI_p3	ATCAAATTTGAGTTCGCGCC	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.7	58.0	842-1069 bp	1.0	77.9
rbcL-psaI_p4	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	867-1094 bp	1.0	77.8
rbcL-psaI_p5	ATCAAATTTGAGTTCGCGCC	AAGGAAGCCATTGCAATTGC	45.0	45.0	57.7	57.9	849-1076 bp	1.0	77.3