Hotspot

atpI-atpH

LSC · 33064-33557 · 494 bp

Back to Homolepis

Pi 0.00406

MarkerSeek score 56.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 2
pi: 0.00406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	562-563 bp	1.0	74.6
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	584-585 bp	1.0	74.5
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	569-570 bp	1.0	74.2
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	TCCAGCAGCAATAACGGAAG	50.0	50.0	57.5	58.3	579-580 bp	1.0	74.1
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	AAGCAGCAGCAATTAGTGGA	45.0	45.0	58.6	57.8	575-576 bp	1.0	74.1