Hotspot

rpoC2

LSC · 19714-23838 · 4125 bp

Back to Homalomena

Pi 0.00097

MarkerSeek score 54.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4125
variable_sites: 4
pi: 0.00097
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTTCCGCTCTCAACAATCCT	ACGCAAGGTCTTCCAAAAGT	50.0	45.0	57.9	57.9	340 bp	1.0	79.2
rpoC2_p2	CTCGTTCCGCTCTCAACAAT	ACGCAAGGTCTTCCAAAAGT	50.0	45.0	58.3	57.9	343 bp	1.0	77.8
rpoC2_p3	TCGTTCCGCTCTCAACAATC	ACGCAAGGTCTTCCAAAAGT	50.0	45.0	58.3	57.9	342 bp	1.0	77.7
rpoC2_p4	GTTCCGCTCTCAACAATCCT	CGCAAGGTCTTCCAAAAGTG	50.0	50.0	57.9	57.6	339 bp	1.0	76.4
rpoC2_p5	CTCGTTCCGCTCTCAACAAT	CGCAAGGTCTTCCAAAAGTG	50.0	50.0	58.3	57.6	342 bp	1.0	74.9