Hotspot

rps15-ycf1

SSC · 133411-134369 · 959 bp

Back to Homalomena

Pi 0.0099

MarkerSeek score 69.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 959
variable_sites: 9
pi: 0.0099
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGACCCTTTGTTTTCT	TGGTAGTCGTTTCAGTATGTCA	38.1	40.9	55.5	56.9	1106-1309 bp	1.0	43.9
rps15-ycf1_p2	TCAACAGACCCTTTGTTTTCT	AGCTTGTATGAATCGGTATTGGT	38.1	39.1	55.5	58.0	1142-1345 bp	1.0	43.8
rps15-ycf1_p3	TCAACAGACCCTTTGTTTTCTTC	AGCTTGTATGAATCGGTATTGGT	39.1	39.1	57.4	58.0	1142-1345 bp	1.0	43.8
rps15-ycf1_p4	CAACAGACCCTTTGTTTTCTTCT	AGCTTGTATGAATCGGTATTGGT	39.1	39.1	57.4	58.0	1141-1344 bp	1.0	43.8
rps15-ycf1_p5	TGAAATTCAACAGACCCTTTGT	AGCTTGTATGAATCGGTATTGGT	36.4	39.1	56.4	58.0	1148-1351 bp	1.0	43.8