Hotspot

ndhG-ndhI

SSC · 128305-128998 · 694 bp

Back to Homalomena

Pi 0.00444

MarkerSeek score 63.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 694
variable_sites: 3
pi: 0.00444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CTACCCCTAAGCCCCCTAAT	TCTTCATTCAAGGACGATTACCA	55.0	39.1	57.6	57.6	801-1006 bp	1.0	51.1
ndhG-ndhI_p2	CTACCCCTAAGCCCCCTAAT	ACTTCTTCATTCAAGGACGA	55.0	40.0	57.6	54.3	804-1009 bp	1.0	49.0
ndhG-ndhI_p3	CTACCCCTAAGCCCCCTAAT	CTTCATTCAAGGACGATTACCA	55.0	40.9	57.6	56.2	800-1005 bp	1.0	47.9
ndhG-ndhI_p4	CTACCCCTAAGCCCCCTAAT	AACTTCTTCATTCAAGGACGA	55.0	38.1	57.6	55.2	805-1010 bp	1.0	47.9
ndhG-ndhI_p5	CTACCCCTAAGCCCCCTAAT	TCATTCAAGGACGATTACCA	55.0	40.0	57.6	54.1	798-1003 bp	1.0	47.1