Hotspot

petA-psbJ

LSC · 70845-72024 · 1180 bp

Back to Homalomena

Pi 0.0136

MarkerSeek score 67.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1180
variable_sites: 16
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGCGTTCAAGGTCTTTTGTT	TCCGGATTAGGCTCATCTCT	45.0	50.0	57.5	57.3	1309-1315 bp	1.0	72.1
petA-psbJ_p2	CGCGTTCAAGGTCTTTTGTT	CCGGATTAGGCTCATCTCTG	45.0	55.0	57.5	57.3	1308-1314 bp	1.0	71.7
petA-psbJ_p3	ACGCGTTCAAGGTCTTTTGT	TCCGGATTAGGCTCATCTCT	45.0	50.0	58.6	57.3	1310-1316 bp	1.0	70.9
petA-psbJ_p4	ACGCGTTCAAGGTCTTTTGT	CCGGATTAGGCTCATCTCTG	45.0	55.0	58.6	57.3	1309-1315 bp	1.0	70.4
petA-psbJ_p5	CGCGTTCAAGGTCTTTTGTT	TTCCGGATTAGGCTCATCTCT	45.0	47.6	57.5	58.0	1310-1316 bp	1.0	69.3