Hotspot

ycf4-cemA

LSC · 67979-68978 · 1000 bp

Back to Homalomena

Pi 0.003

MarkerSeek score 60.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 3
pi: 0.003
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTAGCCTATTTCTTGCGCGT	ATGAGAGAGAGACCCACCAG	45.0	55.0	58.0	57.8	1100-1101 bp	1.0	78.9
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.9	57.8	1087-1088 bp	1.0	78.4
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.1	57.8	1089-1090 bp	1.0	78.1
ycf4-cemA_p4	AAAAGCTGCGGAATTAGCCT	ATGAGAGAGAGACCCACCAG	45.0	55.0	58.2	57.8	1113-1114 bp	1.0	77.7
ycf4-cemA_p5	TTAGCCTATTTCTTGCGCGT	TTCGGATTGCCTGGTATTCC	45.0	50.0	58.0	57.7	1156-1157 bp	1.0	77.4