Hotspot

accD-psaI

LSC · 65584-66945 · 1362 bp

Back to Homalomena

Pi 0.000749

MarkerSeek score 61.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1362
variable_sites: 1
pi: 0.000749
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1545-1552 bp	1.0	81.1
accD-psaI_p2	CCACGGTTTCCTTTCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1450-1457 bp	1.0	81.1
accD-psaI_p3	TCCACGGTTTCCTTTCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1451-1458 bp	1.0	81.1
accD-psaI_p4	GCGGCTGAGTATTCATTCCA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1539-1546 bp	1.0	80.8
accD-psaI_p5	TCACAAGCGGCTGAGTATTC	AGAAACCATTGCAATTGCCG	50.0	45.0	58.0	57.9	1550-1557 bp	1.0	80.6