Hotspot

trnF-GAA-ndhJ

LSC · 54224-55203 · 980 bp

Back to Homalomena

Pi 0.00105

MarkerSeek score 61.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1079-1090 bp	1.0	80.5
trnF-GAA-ndhJ_p2	AAGATAAGGCGATGGCATGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1144-1155 bp	1.0	80.0
trnF-GAA-ndhJ_p3	GATAAGGCGATGGCATGGAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1142-1153 bp	1.0	80.0
trnF-GAA-ndhJ_p4	TAAGGCGATGGCATGGAAAA	TGAAAGTTGGATAGGCTGGC	45.0	50.0	57.9	57.9	1140-1151 bp	1.0	79.7
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	1121-1132 bp	1.0	79.2