Hotspot

trnT-UGU-trnL-UAA

LSC · 51897-53175 · 1279 bp

Back to Homalomena

Pi 0.000811
MarkerSeek score 62.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1279
variable_sites
1
pi
0.000811
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAAAAAGCCAGCTATCGGA ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1378-1627 bp 1.0 82.2
trnT-UGU-trnL-UAA_p2 AGGGAAAAAGCCAGCTATCG ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1380-1629 bp 1.0 82.1
trnT-UGU-trnL-UAA_p3 GGAAAAAGCCAGCTATCGGA TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1417-1666 bp 1.0 81.7
trnT-UGU-trnL-UAA_p4 AGGGAAAAAGCCAGCTATCG TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1419-1668 bp 1.0 81.6
trnT-UGU-trnL-UAA_p5 GGAAAAAGCCAGCTATCGGA TAGCGTCTACCAATTTCGCC 50.0 50.0 58.0 58.1 1386-1635 bp 1.0 81.6