Hotspot

ycf3-trnS-GGA

LSC · 49258-50155 · 898 bp

Back to Homalomena

Pi 0.00112

MarkerSeek score 60.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 1
pi: 0.00112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGGCTACAACTGAAGAGGTC	CCCTCGGTAAACAAAAGCCT	50.0	50.0	56.9	58.1	1017-1023 bp	1.0	70.7
ycf3-trnS-GGA_p2	TGGCTACAACTGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	56.9	58.2	1038-1044 bp	1.0	70.3
ycf3-trnS-GGA_p3	TGGCTACAACTGAAGAGGTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	56.9	57.8	988-994 bp	1.0	70.0
ycf3-trnS-GGA_p4	TGGCTACAACTGAAGAGGTCT	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.4	58.1	1017-1023 bp	1.0	68.7
ycf3-trnS-GGA_p5	TTGGCTACAACTGAAGAGGTC	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.6	58.1	1018-1024 bp	1.0	68.6