Hotspot

psbM-trnD-GUC

LSC · 34112-34717 · 606 bp

Back to Homalomena

Pi 0.00166

MarkerSeek score 60.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.00166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	717-719 bp	1.0	61.8
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	CCGCTGGGATTGTAGTTCAA	45.0	50.0	55.8	57.9	730-732 bp	1.0	61.6
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	ATGTATGCTGTACATCCCGC	45.0	50.0	55.8	57.8	746-748 bp	1.0	61.1
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	724-726 bp	1.0	58.7
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	GTATGCTGTACATCCCGCTG	45.0	55.0	55.8	58.5	744-746 bp	1.0	58.5