Hotspot

rps19

IRa · 156041-156319 · 279 bp

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Pi 0.00239

MarkerSeek score 32.40

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRa
length_bp: 279
variable_sites: 1
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTGATTCTTCGTCGCCGTAG	TGAACGATAAGCATCTATATTTTCCT	50.0	30.8	58.1	56.6	432 bp	1.0	40.2
rps19_p2	ATTTGATTCTTCGTCGCCGT	TGAACGATAAGCATCTATATTTTCCT	45.0	30.8	58.3	56.6	434 bp	1.0	40.2
rps19_p3	TTTGATTCTTCGTCGCCGTA	TGAACGATAAGCATCTATATTTTCCT	45.0	30.8	57.6	56.6	433 bp	1.0	40.2
rps19_p4	TTGATTCTTCGTCGCCGTAG	TGAACGATAAGCATCTATATTTTCC	50.0	32.0	58.1	55.4	432 bp	1.0	40.2
rps19_p5	TTGATTCTTCGTCGCCGTAG	TGAACGATAAGCATCTATATTTTCCTT	50.0	29.6	58.1	57.2	432 bp	1.0	40.2