Hotspot

rpl22

LSC · 84289-84678 · 390 bp

Back to Homalium

Pi 0.012

MarkerSeek score 35.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 390
variable_sites: 7
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGCAAGAATATTCGGGTGCA	TCCCGGGCATCTACCATTAT	45.0	50.0	57.9	58.0	1224-1252 bp	1.0	82.0
rpl22_p2	GTTTGTAGAGGAACCCTGCC	TCCCGGGCATCTACCATTAT	55.0	50.0	58.2	58.0	1403-1431 bp	1.0	81.1
rpl22_p3	TGCAAGAATATTCGGGTGCA	TTGATTCTTCGTCGCCGTAG	45.0	50.0	57.9	58.1	1419-1447 bp	1.0	81.1
rpl22_p4	TTTCCATCAAGACGTCCTGC	TCCCGGGCATCTACCATTAT	50.0	50.0	58.2	58.0	1352-1380 bp	1.0	81.1
rpl22_p5	GTTTGTAGAGGAACCCTGCC	TTGATTCTTCGTCGCCGTAG	55.0	50.0	58.2	58.1	1598-1626 bp	1.0	80.3