Hotspot

trnR-trnN

IRb · 109459-110129 · 671 bp

Back to Homalium

Pi 0.00499

MarkerSeek score 66.10

Primer available yes

Label trnR-trnN

Feature Metadata

label_name: trnR-trnN
feature_type: igs
region: IRb
length_bp: 671
variable_sites: 5
pi: 0.00499
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnN_p1	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.1	58.0	825-850 bp	1.0	82.2
trnR-trnN_p2	TAGGGTATTCCGGGGAACAG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.2	58.0	840-865 bp	1.0	81.2
trnR-trnN_p3	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	789-814 bp	1.0	81.2
trnR-trnN_p4	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	50.0	50.0	58.1	57.8	789-814 bp	1.0	81.2
trnR-trnN_p5	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	776-801 bp	1.0	81.2