Hotspot

petA-psbJ

LSC · 62898-63856 · 959 bp

Back to Homalium

Pi 0.0146

MarkerSeek score 60.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 959
variable_sites: 21
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1103-1291 bp	1.0	80.4
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1105-1293 bp	1.0	80.4
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1097-1285 bp	1.0	79.2
petA-psbJ_p4	CCATTACGTGTCCAAGGTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	1102-1290 bp	1.0	76.4
petA-psbJ_p5	CCCATTACGTGTCCAAGGTC	ATGGCTCCTATTCCGGATTG	55.0	50.0	58.3	57.1	1112-1300 bp	1.0	76.4