Hotspot

ycf4-cemA

LSC · 60609-61007 · 399 bp

Back to Homalium

Pi 0.0623

MarkerSeek score 75.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 30
pi: 0.0623
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	CCACCAGGGCAAAAATACTA	45.0	45.0	58.1	55.3	367-501 bp	1.0	67.3
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	AGAAGGGGAATGAATGCTTT	45.0	40.0	58.1	54.9	333-467 bp	1.0	64.2
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CCACCAGGGCAAAAATACTAT	45.0	42.9	58.1	55.9	367-501 bp	1.0	63.9
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	AGAAGGGGAATGAATGCTTTT	45.0	38.1	58.1	55.7	333-467 bp	1.0	62.9
ycf4-cemA_p5	AGCGGAATTGGCCTATTTCT	CCACCAGGGCAAAAATACTA	45.0	45.0	57.3	55.3	374-508 bp	1.0	62.0