Hotspot

trnF-ndhJ

LSC · 47731-48495 · 765 bp

Back to Homalium

Pi 0.00761

MarkerSeek score 66.90

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 8
pi: 0.00761
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	814-878 bp	1.0	84.0
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	813-877 bp	1.0	84.0
trnF-ndhJ_p3	CAGGTAGAGCAGAGGACTGA	CCGGAAAGTTGGATAGGGTG	55.0	55.0	57.9	58.0	838-902 bp	1.0	83.3
trnF-ndhJ_p4	CAGGTAGAGCAGAGGACTGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	57.9	58.0	837-901 bp	1.0	83.3
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	811-875 bp	1.0	82.5