Hotspot

atpH-atpI

LSC · 12480-13507 · 1028 bp

Back to Homalium

Pi 0.011

MarkerSeek score 54.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 17
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GTGAATCCTTGGAGGGACAC	45.0	55.0	58.0	57.9	1086-1242 bp	1.0	83.3
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	GTGAATCCTTGGAGGGACAC	45.0	55.0	57.9	57.9	1078-1234 bp	1.0	83.2
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1115-1271 bp	1.0	83.1
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1107-1263 bp	1.0	82.9
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	GTGAATCCTTGGAGGGACAC	50.0	55.0	57.9	57.9	1124-1280 bp	1.0	82.5