Hotspot

matK-trnK

LSC · 3612-4377 · 766 bp

Back to Homalium

Pi 0.021

MarkerSeek score 65.20

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 18
pi: 0.021
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	TTCTTTGAGATTTCTCTATTTTCA	TGGGGTTGCTAACTCAATGG	25.0	50.0	52.4	57.8	756-837 bp	1.0	45.8
matK-trnK_p2	TTCTTTGAGATTTCTCTATTTTCA	GGGGTTGCTAACTCAATGGT	25.0	50.0	52.4	57.8	755-836 bp	1.0	45.8
matK-trnK_p3	TCTTTGAGATTTCTCTATTTTCAT	TGGGGTTGCTAACTCAATGG	25.0	50.0	52.2	57.8	755-836 bp	1.0	45.8
matK-trnK_p4	TCTTTGAGATTTCTCTATTTTCAT	GGGGTTGCTAACTCAATGGT	25.0	50.0	52.2	57.8	754-835 bp	1.0	45.8
matK-trnK_p5	TCTAAATTTCTTTGAGATTTCTCT	TGGGGTTGCTAACTCAATGG	25.0	50.0	52.1	57.8	763-844 bp	1.0	45.8