Hotspot

rps16

LSC · 5365-6557 · 1193 bp

Back to Holoptelea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1193
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAACGACGACATTACCCTCT	TGTACCAACTGAACCAATGACT	45.0	40.9	56.6	57.6	1306 bp	1.0	49.2
rps16_p2	AACGACGACATTACCCTCTT	TGTACCAACTGAACCAATGACT	45.0	40.9	56.6	57.6	1305 bp	1.0	49.2
rps16_p3	ACGACGACATTACCCTCTTT	TGTACCAACTGAACCAATGACT	45.0	40.9	56.6	57.6	1304 bp	1.0	49.2
rps16_p4	AACGACGACATTACCCTCTTT	TGTACCAACTGAACCAATGACT	42.9	40.9	57.3	57.6	1305 bp	1.0	47.2
rps16_p5	ACGACGACATTACCCTCTTTT	TGTACCAACTGAACCAATGACT	42.9	40.9	57.3	57.6	1304 bp	1.0	47.2