Hotspot

matK

LSC · 2130-3653 · 1524 bp

Back to Holoptelea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTTCGATTTCCATGGTTGCA	CGTTTCCGGTTCAAATCGAA	40.0	45.0	56.2	57.0	1577 bp	1.0	58.2
matK_p2	TTTCGATTTCCATGGTTGCA	ATCTCCTAGCTCCGTTTCCG	40.0	55.0	56.2	59.0	1589 bp	1.0	58.1
matK_p3	TTTCGATTTCCATGGTTGCA	ACCTTGTTTTGGCTGTATCGT	40.0	42.9	56.2	58.1	1637 bp	1.0	56.7
matK_p4	TTCGATTTCCATGGTTGCAT	CGTTTCCGGTTCAAATCGAA	40.0	45.0	56.0	57.0	1576 bp	1.0	56.3
matK_p5	TTCGATTTCCATGGTTGCAT	ATCTCCTAGCTCCGTTTCCG	40.0	55.0	56.0	59.0	1588 bp	1.0	56.2