Hotspot

atpA

LSC · 11144-12667 · 1524 bp

Back to Holoptelea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ACAAAAGATTCTCTCAAATTCTAA	TGGCATGTTTGGAACGATGA	25.0	45.0	52.6	58.1	1731 bp	1.0	40.0
atpA_p2	TTACAAAAGATTCTCTCAAATTCT	TGGCATGTTTGGAACGATGA	25.0	45.0	52.6	58.1	1733 bp	1.0	40.0
atpA_p3	ACAAAAGATTCTCTCAAATTCTAA	TTGGCATGTTTGGAACGATG	25.0	45.0	52.6	57.3	1732 bp	1.0	40.0
atpA_p4	TTACAAAAGATTCTCTCAAATTCT	TTGGCATGTTTGGAACGATG	25.0	45.0	52.6	57.3	1734 bp	1.0	40.0
atpA_p5	ACAAAAGATTCTCTCAAATTCTAA	GGCATGTTTGGAACGATGAA	25.0	45.0	52.6	57.0	1730 bp	1.0	40.0