Hotspot

atpH-atpI

LSC · 14731-15990 · 1260 bp

Back to Holoptelea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1260
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATCCCTTCTACAGCTT	ACCGCCGCTTATATAGGAGA	50.0	50.0	58.0	57.8	1422 bp	1.0	78.1
atpH-atpI_p2	CGCAATCCCTTCTACAGCTT	TTTTTGCAACCTTAACCGCC	50.0	45.0	58.0	57.8	1436 bp	1.0	77.9
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	ACCGCCGCTTATATAGGAGA	50.0	50.0	57.8	57.8	1342 bp	1.0	77.0
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TTTTTGCAACCTTAACCGCC	50.0	45.0	57.8	57.8	1356 bp	1.0	76.8
atpH-atpI_p5	AATCCCTTCTACAGCTTGGC	ACCGCCGCTTATATAGGAGA	50.0	50.0	57.6	57.8	1419 bp	1.0	74.9