Hotspot

trnS-GCU-trnG-GCC

LSC · 8789-9617 · 829 bp

Back to Holoptelea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	GTTTTTGCTCGGAGTGGGAT	50.0	50.0	58.0	58.5	963 bp	1.0	76.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	GTTTTTGCTCGGAGTGGGAT	50.0	50.0	58.0	58.5	962 bp	1.0	76.2
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	GTTTTTGCTCGGAGTGGGAT	50.0	50.0	58.2	58.5	1017 bp	1.0	75.1
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	AAGTTTTTGCTCGGAGTGGG	50.0	50.0	58.0	58.7	965 bp	1.0	74.5
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	AAGTTTTTGCTCGGAGTGGG	50.0	50.0	58.0	58.7	964 bp	1.0	74.5