Hotspot

rps16

LSC · 4494-5585 · 1092 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1092
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GGATTGGCACGACATAAATCC TGCTCCATAGACAGCAAGTT 47.6 45.0 57.4 56.8 1327 bp 1.0 57.7
rps16_p2 GATTGGCACGACATAAATCCA TGCTCCATAGACAGCAAGTT 42.9 45.0 56.7 56.8 1326 bp 1.0 52.1
rps16_p3 TGGCACGACATAAATCCAAA TGCTCCATAGACAGCAAGTT 40.0 45.0 55.6 56.8 1323 bp 1.0 51.9
rps16_p4 TTGGCACGACATAAATCCAA TGCTCCATAGACAGCAAGTT 40.0 45.0 55.6 56.8 1324 bp 1.0 51.9
rps16_p5 TTGGCACGACATAAATCCAAA TGCTCCATAGACAGCAAGTT 38.1 45.0 56.4 56.8 1324 bp 1.0 50.2