Hotspot

rpl16-rps3

LSC · 78730-78873 · 144 bp

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Pi 0
MarkerSeek score 53.20
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
144
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AACGAGTCACACACTAAGCA GGGTAGACTTCCCCTCCAAA 45.0 55.0 57.1 58.3 279-280 bp 1.0 70.5
rpl16-rps3_p2 ACGAGTCACACACTAAGCAT GGGTAGACTTCCCCTCCAAA 45.0 55.0 56.9 58.3 278-279 bp 1.0 68.7
rpl16-rps3_p3 AACGAGTCACACACTAAGCA TTCCCCTCCAAACAATTCGC 45.0 50.0 57.1 58.7 271-272 bp 1.0 67.3
rpl16-rps3_p4 AACGAGTCACACACTAAGCA GGTAGACTTCCCCTCCAAAC 45.0 55.0 57.1 57.2 278-279 bp 1.0 67.0
rpl16-rps3_p5 AACGAGTCACACACTAAGCAT GGGTAGACTTCCCCTCCAAA 42.9 55.0 57.6 58.3 279-280 bp 1.0 66.5