Hotspot

rps8-rpl14

LSC · 76747-76889 · 143 bp

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Pi 0
MarkerSeek score 53.20
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
143
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TCAGCAATAGTGTCCTTGCC GCGATTGCCGAAGAATTGAG 50.0 50.0 57.9 57.9 234-235 bp 1.0 78.7
rps8-rpl14_p2 GCAATAGTGTCCTTGCCCAT GCGATTGCCGAAGAATTGAG 50.0 50.0 58.2 57.9 231-232 bp 1.0 77.7
rps8-rpl14_p3 TCAGCAATAGTGTCCTTGCC AGGAACTCGAGTTTTTGGCG 50.0 50.0 57.9 58.8 253-254 bp 1.0 73.2
rps8-rpl14_p4 CAGCAATAGTGTCCTTGCCC GCGATTGCCGAAGAATTGAG 55.0 50.0 58.9 57.9 233-234 bp 1.0 72.4
rps8-rpl14_p5 GCAATAGTGTCCTTGCCCAT AGGAACTCGAGTTTTTGGCG 50.0 50.0 58.2 58.8 250-251 bp 1.0 72.3