Hotspot

ycf4-cemA

LSC · 58114-58563 · 450 bp

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Pi 0
MarkerSeek score 55.80
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
450
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA CCCAGGGCAAAAGTACGATA 45.0 50.0 57.9 57.3 540 bp 1.0 73.9
ycf4-cemA_p2 CTTGCGCGTACCAATTGAAG CCCAGGGCAAAAGTACGATA 50.0 50.0 58.1 57.3 542 bp 1.0 73.6
ycf4-cemA_p3 ACAAAAAGCTGCCGAATTGG CCCAGGGCAAAAGTACGATA 45.0 50.0 57.8 57.3 569 bp 1.0 73.3
ycf4-cemA_p4 TTTCTTGCGCGTACCAATTG CCCAGGGCAAAAGTACGATA 45.0 50.0 57.7 57.3 545 bp 1.0 71.9
ycf4-cemA_p5 CCTATTTCTTGCGCGTACCA CCCAGGGCAAAAGTACGATA 50.0 50.0 58.4 57.3 549 bp 1.0 71.7