Hotspot

petN-trnC-GCA

LSC · 17182-18090 · 909 bp

Back to Holcus

Pi 0.0011
MarkerSeek score 59.50
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
909
variable_sites
1
pi
0.0011
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 957-958 bp 1.0 70.5
petN-trnC-GCA_p2 AGCCCAAGCGAGACTTACTA CCAGTTCAAATCTGGGTGCC 50.0 55.0 58.2 59.1 959-960 bp 1.0 70.1
petN-trnC-GCA_p3 CCACTCCTCCCCCATACTAC AGTTCAAATCTGGGTGCCG 60.0 52.6 58.3 58.1 1013-1014 bp 1.0 69.5
petN-trnC-GCA_p4 CCACTCCTCCCCCATACTAC CCAGTTCAAATCTGGGTGCC 60.0 55.0 58.3 59.1 1015-1016 bp 1.0 69.0
petN-trnC-GCA_p5 TCCACTCCTCCCCCATACTA AGTTCAAATCTGGGTGCCG 55.0 52.6 58.4 58.1 1014-1015 bp 1.0 68.8