Hotspot

psbZ-trnG-GCC

LSC · 11865-12150 · 286 bp

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Pi 0.0035
MarkerSeek score 54.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
286
variable_sites
1
pi
0.0035
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG TACCATTGAACTACGCTCGC 50.0 50.0 57.8 58.1 416 bp 1.0 77.9
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTC TACCATTGAACTACGCTCGC 55.0 50.0 58.3 58.1 414 bp 1.0 77.5
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG TAAATTAAGTGGGCGAGCGG 50.0 50.0 57.8 58.3 478 bp 1.0 75.8
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC TAAATTAAGTGGGCGAGCGG 55.0 50.0 58.3 58.3 476 bp 1.0 75.4
psbZ-trnG-GCC_p5 TTGCTTCTCCTGATGGTTGG TCGAACCCGTATCTTCTCCT 50.0 50.0 57.8 57.6 452 bp 1.0 75.1