Hotspot

matK-trnK-UUU

LSC · 3225-3893 · 669 bp

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Pi 0
MarkerSeek score 58.90
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
669
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CCACAATCATGGGCAAATGC AATGTTTTGACAGAAGCGCG 50.0 45.0 58.3 58.0 889-890 bp 1.0 77.1
matK-trnK-UUU_p2 CACAATCATGGGCAAATGCA AATGTTTTGACAGAAGCGCG 45.0 45.0 57.6 58.0 888-889 bp 1.0 76.7
matK-trnK-UUU_p3 CCACAATCATGGGCAAATGC CGCGCAGTGTAATTCCATTG 50.0 50.0 58.3 58.2 873-874 bp 1.0 75.9
matK-trnK-UUU_p4 CACAATCATGGGCAAATGCA CGCGCAGTGTAATTCCATTG 45.0 50.0 57.6 58.2 872-873 bp 1.0 75.5
matK-trnK-UUU_p5 CACAATCATGGGCAAATGCAT AATGTTTTGACAGAAGCGCG 42.9 45.0 58.1 58.0 888-889 bp 1.0 71.1