primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	ACCCAATATCTTGCTAGAACAAG	TACGTATGCTTGGGAGTCCT	23	20	39.1	50.0	56.3	57.8	1.0	9.6	4.829949	87	1148	1192-1192	1.000000	41.4
psbA_p2	psbA	2	ACCCAATATCTTGCTAGAACA	TACGTATGCTTGGGAGTCCT	21	20	38.1	50.0	54.3	57.8	1.0	9.6	4.836556	87	1148	1192-1192	1.000000	41.3
psbA_p3	psbA	3	TGCTAGAACAAGATATTGGGT	TACGTATGCTTGGGAGTCCT	21	20	38.1	50.0	54.3	57.8	4.9	9.6	4.836556	87	1148	1181-1181	1.000000	41.3
psbA_p4	psbA	4	ACCCAATATCTTGCTAGAACAA	TACGTATGCTTGGGAGTCCT	22	20	36.4	50.0	55.1	57.8	1.0	9.6	5.009846	87	1148	1192-1192	1.000000	40.0
psbA_p5	psbA	5	TTGCTAGAACAAGATATTGGGT	TACGTATGCTTGGGAGTCCT	22	20	36.4	50.0	55.1	57.8	4.9	9.6	5.009846	87	1148	1182-1182	1.000000	40.0
trnK-UUU_p1	trnK-UUU	1	TGTCAACCAAATCAGCTCAA	AATGTTTTGACAGAAGCGCG	20	20	40.0	45.0	55.5	58.0	0.0	0.0	2.557431	1383	3930	2743-2744	1.000000	59.6
trnK-UUU_p2	trnK-UUU	2	TGTCAACCAAATCAGCTCAAAG	AATGTTTTGACAGAAGCGCG	22	20	40.9	45.0	57.4	58.0	0.0	0.0	2.609541	1383	3930	2743-2744	1.000000	59.2
trnK-UUU_p3	trnK-UUU	3	TGTCAACCAAATCAGCTCAA	CGCGCAGTGTAATTCCATTG	20	20	40.0	50.0	55.5	58.2	0.0	8.7	2.706979	1383	3930	2727-2728	1.000000	58.4
trnK-UUU_p4	trnK-UUU	4	TGTCAACCAAATCAGCTCAAAG	CGCGCAGTGTAATTCCATTG	22	20	40.9	50.0	57.4	58.2	0.0	8.7	2.759088	1383	3930	2727-2728	1.000000	58.0
trnK-UUU_p5	trnK-UUU	5	TGTCAACCAAATCAGCTCAAA	AATGTTTTGACAGAAGCGCG	21	20	38.1	45.0	56.3	58.0	0.0	0.0	2.760363	1383	3930	2743-2744	1.000000	57.9
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCACAATCATGGGCAAATGC	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.377563	3225	3893	889-890	1.000000	77.1
matK-trnK-UUU_p2	matK-trnK-UUU	2	CACAATCATGGGCAAATGCA	AATGTTTTGACAGAAGCGCG	20	20	45.0	45.0	57.6	58.0	7.8	0.0	0.424867	3225	3893	888-889	1.000000	76.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCACAATCATGGGCAAATGC	CGCGCAGTGTAATTCCATTG	20	20	50.0	50.0	58.3	58.2	0.0	8.7	0.527110	3225	3893	873-874	1.000000	75.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	CACAATCATGGGCAAATGCA	CGCGCAGTGTAATTCCATTG	20	20	45.0	50.0	57.6	58.2	7.8	8.7	0.574414	3225	3893	872-873	1.000000	75.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	CACAATCATGGGCAAATGCAT	AATGTTTTGACAGAAGCGCG	21	20	42.9	45.0	58.1	58.0	7.8	0.0	1.123786	3225	3893	888-889	1.000000	71.1
rps16_p1	rps16	1	GGATTGGCACGACATAAATCC	TGCTCCATAGACAGCAAGTT	21	20	47.6	45.0	57.4	56.8	0.0	0.0	2.784695	4494	5585	1327-1327	1.000000	57.7
rps16_p2	rps16	2	GATTGGCACGACATAAATCCA	TGCTCCATAGACAGCAAGTT	21	20	42.9	45.0	56.7	56.8	0.0	0.0	3.487224	4494	5585	1326-1326	1.000000	52.1
rps16_p3	rps16	3	TGGCACGACATAAATCCAAA	TGCTCCATAGACAGCAAGTT	20	20	40.0	45.0	55.6	56.8	0.0	0.0	3.516216	4494	5585	1323-1323	1.000000	51.9
rps16_p4	rps16	4	TTGGCACGACATAAATCCAA	TGCTCCATAGACAGCAAGTT	20	20	40.0	45.0	55.6	56.8	0.0	0.0	3.516216	4494	5585	1324-1324	1.000000	51.9
rps16_p5	rps16	5	TTGGCACGACATAAATCCAAA	TGCTCCATAGACAGCAAGTT	21	20	38.1	45.0	56.4	56.8	0.0	0.0	3.729953	4494	5585	1324-1324	1.000000	50.2
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	CAACCACTCAGCCATCTCTC	GGCTGAGGAAGTCGCAATTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.045531	11324	11675	427-427	1.000000	79.6
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	GAGCTATCAACCACTCAGCC	GGCTGAGGAAGTCGCAATTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.077431	11324	11675	434-434	1.000000	79.4
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	GGAGCTATCAACCACTCAGC	GGCTGAGGAAGTCGCAATTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.077431	11324	11675	435-435	1.000000	79.4
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	CAAGACCGGAGCTATCAACC	GGCTGAGGAAGTCGCAATTA	20	20	55.0	50.0	58.1	58.0	9.5	0.0	0.082851	11324	11675	442-442	1.000000	79.3
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.226377	11324	11675	472-472	1.000000	78.2
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTGCTTCTCCTGATGGTTGG	TACCATTGAACTACGCTCGC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.287750	11865	12150	416-416	1.000000	77.9
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	GCTTCTCCTGATGGTTGGTC	TACCATTGAACTACGCTCGC	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.338732	11865	12150	414-414	1.000000	77.5
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTGCTTCTCCTGATGGTTGG	TAAATTAAGTGGGCGAGCGG	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.546029	11865	12150	478-478	1.000000	75.8
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	TAAATTAAGTGGGCGAGCGG	20	20	55.0	50.0	58.3	58.3	0.0	0.0	0.597010	11865	12150	476-476	1.000000	75.4
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.636976	11865	12150	452-452	1.000000	75.1
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	17182	18090	957-958	1.000000	70.5
petN-trnC-GCA_p2	petN-trnC-GCA	2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	20	20	50.0	55.0	58.2	59.1	0.0	4.3	1.260665	17182	18090	959-960	1.000000	70.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	20	19	60.0	52.6	58.3	58.1	0.0	0.0	1.334774	17182	18090	1013-1014	1.000000	69.5
petN-trnC-GCA_p4	petN-trnC-GCA	4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	20	20	60.0	55.0	58.3	59.1	0.0	4.3	1.391037	17182	18090	1015-1016	1.000000	69.0
petN-trnC-GCA_p5	petN-trnC-GCA	5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.4	58.1	0.0	0.0	1.425206	17182	18090	1014-1015	1.000000	68.8
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	20	20	50.0	50.0	58.1	58.0	14.6	0.0	0.109519	18162	19339	1246-1247	1.000000	79.3
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.250044	18162	19339	1253-1254	1.000000	78.2
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	TGTCAAGTTCTTCTGCCAGG	20	20	50.0	50.0	58.1	57.7	14.6	0.0	0.331976	18162	19339	1329-1330	1.000000	77.5
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18162	19339	1261-1262	1.000000	77.0
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18162	19339	1260-1261	1.000000	77.0
rpoC2_p1	rpoC2	1	CATTGCCGCTCGAAATCAAG	CCTACCGCTTCTCCCAATTC	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.226223	25667	25786	198-198	1.000000	78.6
rpoC2_p2	rpoC2	2	TTGCCGCTCGAAATCAAGAT	CCTACCGCTTCTCCCAATTC	20	20	45.0	55.0	58.3	58.0	0.0	0.0	0.307235	25667	25786	196-196	1.000000	78.0
rpoC2_p3	rpoC2	3	ATTGCCGCTCGAAATCAAGA	CCTACCGCTTCTCCCAATTC	20	20	45.0	55.0	58.3	58.0	0.0	0.0	0.307235	25667	25786	197-197	1.000000	77.9
rpoC2_p4	rpoC2	4	CATTGCCGCTCGAAATCAAG	ATACCTACCGCTTCTCCCAA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.351162	25667	25786	201-201	1.000000	77.6
rpoC2_p5	rpoC2	5	CATTGCCGCTCGAAATCAAG	TACCTACCGCTTCTCCCAAT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.351162	25667	25786	200-200	1.000000	77.6
atpI-atpH_p1	atpI-atpH	1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31263	31840	667-668	1.000000	78.9
atpI-atpH_p2	atpI-atpH	2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31263	31840	670-671	1.000000	78.9
atpI-atpH_p3	atpI-atpH	3	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	20	20	45.0	55.0	57.9	58.2	0.0	0.0	0.253472	31263	31840	724-725	1.000000	78.1
atpI-atpH_p4	atpI-atpH	4	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	20	20	45.0	45.0	57.9	57.8	0.0	3.2	0.268178	31263	31840	657-658	1.000000	78.0
atpI-atpH_p5	atpI-atpH	5	TTTTGCAACATTAGCCGCAG	GCCAATCCAGCAGCAATAAC	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.277543	31263	31840	679-680	1.000000	77.9
rbcL-psaI_p1	rbcL-psaI	1	AGTTCGAGCCGGTAGATACT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.7	57.9	1.9	26.9	0.422537	55732	57139	1511-1511	1.000000	76.8
rbcL-psaI_p2	rbcL-psaI	2	GGCGATCAAATTCGAGTTCG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.6	57.9	0.0	26.9	0.430670	55732	57139	1525-1525	1.000000	76.7
rbcL-psaI_p3	rbcL-psaI	3	TGGAAGGCGATCAAATTCGA	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	57.6	57.9	0.0	26.9	0.472342	55732	57139	1530-1530	1.000000	76.4
rbcL-psaI_p4	rbcL-psaI	4	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.5	57.9	11.3	26.9	0.534920	55732	57139	1573-1573	1.000000	75.9
rbcL-psaI_p5	rbcL-psaI	5	CAGCTTGCAAATGGAGTCCT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.5	57.9	11.3	26.9	0.534920	55732	57139	1574-1574	1.000000	75.9
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	CCCAGGGCAAAAGTACGATA	20	20	45.0	50.0	57.9	57.3	8.4	0.0	0.784317	58114	58563	540-540	1.000000	73.9
ycf4-cemA_p2	ycf4-cemA	2	CTTGCGCGTACCAATTGAAG	CCCAGGGCAAAAGTACGATA	20	20	50.0	50.0	58.1	57.3	8.4	0.0	0.820394	58114	58563	542-542	1.000000	73.6
ycf4-cemA_p3	ycf4-cemA	3	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATA	20	20	45.0	50.0	57.8	57.3	0.0	0.0	0.855958	58114	58563	569-569	1.000000	73.3
ycf4-cemA_p4	ycf4-cemA	4	TTTCTTGCGCGTACCAATTG	CCCAGGGCAAAAGTACGATA	20	20	45.0	50.0	57.7	57.3	8.4	0.0	1.026531	58114	58563	545-545	1.000000	71.9
ycf4-cemA_p5	ycf4-cemA	5	CCTATTTCTTGCGCGTACCA	CCCAGGGCAAAAGTACGATA	20	20	50.0	50.0	58.4	57.3	8.4	0.0	1.055246	58114	58563	549-549	1.000000	71.7
clpP_p1	clpP	1	TCGAAGAAAACCCGAATTTGG	ACTGCGGATTCTTTCTTTCTCT	21	22	42.9	40.9	57.1	57.7	4.5	0.0	4.186869	67341	67991	790-790	1.000000	46.6
clpP_p2	clpP	2	TCGAAGAAAACCCGAATTTGG	GCGGATTCTTTCTTTCTCTTCC	21	22	42.9	45.5	57.1	57.4	4.5	0.0	4.515967	67341	67991	787-787	1.000000	44.0
clpP_p3	clpP	3	TCGAAGAAAACCCGAATTTGG	ACTGCGGATTCTTTCTTTCTC	21	21	42.9	42.9	57.1	56.3	4.5	0.0	4.641496	67341	67991	790-790	1.000000	43.0
clpP_p4	clpP	4	CGAAGAAAACCCGAATTTGG	ACTGCGGATTCTTTCTTTCTCT	20	22	45.0	40.9	55.6	57.7	4.5	0.0	4.711939	67341	67991	789-789	1.000000	42.4
clpP_p5	clpP	5	ATCGAAGAAAACCCGAATTTGG	ACTGCGGATTCTTTCTTTCTCT	22	22	40.9	40.9	57.6	57.7	4.5	0.0	4.713688	67341	67991	791-791	1.000000	42.4
rps8-rpl14_p1	rps8-rpl14	1	TCAGCAATAGTGTCCTTGCC	GCGATTGCCGAAGAATTGAG	20	20	50.0	50.0	57.9	57.9	3.8	0.0	0.210659	76747	76889	234-235	1.000000	78.7
rps8-rpl14_p2	rps8-rpl14	2	GCAATAGTGTCCTTGCCCAT	GCGATTGCCGAAGAATTGAG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.329040	76747	76889	231-232	1.000000	77.7
rps8-rpl14_p3	rps8-rpl14	3	TCAGCAATAGTGTCCTTGCC	AGGAACTCGAGTTTTTGGCG	20	20	50.0	50.0	57.9	58.8	3.8	30.2	0.887114	76747	76889	253-254	1.000000	73.2
rps8-rpl14_p4	rps8-rpl14	4	CAGCAATAGTGTCCTTGCCC	GCGATTGCCGAAGAATTGAG	20	20	55.0	50.0	58.9	57.9	3.8	0.0	0.998749	76747	76889	233-234	1.000000	72.4
rps8-rpl14_p5	rps8-rpl14	5	GCAATAGTGTCCTTGCCCAT	AGGAACTCGAGTTTTTGGCG	20	20	50.0	50.0	58.2	58.8	0.0	30.2	1.005495	76747	76889	250-251	1.000000	72.3
rpl16-rps3_p1	rpl16-rps3	1	AACGAGTCACACACTAAGCA	GGGTAGACTTCCCCTCCAAA	20	20	45.0	55.0	57.1	58.3	0.0	5.9	1.217790	78730	78873	279-280	1.000000	70.5
rpl16-rps3_p2	rpl16-rps3	2	ACGAGTCACACACTAAGCAT	GGGTAGACTTCCCCTCCAAA	20	20	45.0	55.0	56.9	58.3	0.0	5.9	1.444078	78730	78873	278-279	1.000000	68.7
rpl16-rps3_p3	rpl16-rps3	3	AACGAGTCACACACTAAGCA	TTCCCCTCCAAACAATTCGC	20	20	45.0	50.0	57.1	58.7	0.0	0.0	1.629827	78730	78873	271-272	1.000000	67.3
rpl16-rps3_p4	rpl16-rps3	4	AACGAGTCACACACTAAGCA	GGTAGACTTCCCCTCCAAAC	20	20	45.0	55.0	57.1	57.2	0.0	0.0	1.666536	78730	78873	278-279	1.000000	67.0
rpl16-rps3_p5	rpl16-rps3	5	AACGAGTCACACACTAAGCAT	GGGTAGACTTCCCCTCCAAA	21	20	42.9	55.0	57.6	58.3	0.0	5.9	1.721631	78730	78873	279-280	1.000000	66.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	105030	105647	717-717	1.000000	79.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	105030	105647	716-716	1.000000	79.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGTTTTTCTGGGCAACA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.185902	105030	105647	720-720	1.000000	78.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAACAA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.185902	105030	105647	719-719	1.000000	78.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	105030	105647	689-689	1.000000	78.1