Hotspot

trnN-GUU-rpl32

IRb · 109673-110425 · 753 bp

Back to Holcoglossum

Pi 0.047
MarkerSeek score 85.30
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
IRb
length_bp
753
variable_sites
83
pi
0.047
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 58.1 57.3 783-943 bp 1.0 60.9
trnN-GUU-rpl32_p2 ACGACCAGTCAGTTAACAGC GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 57.8 57.3 789-949 bp 1.0 60.6
trnN-GUU-rpl32_p3 AGGATTCGAACCTACGACCA GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 58.2 57.3 802-962 bp 1.0 60.5
trnN-GUU-rpl32_p4 AGTCAGTTAACAGCCAACCG AGAAAAAGCTTTTACTGCCTCT 50.0 36.4 58.1 56.2 790-950 bp 1.0 60.2
trnN-GUU-rpl32_p5 GGATTCGAACCTACGACCAG GCTTTTACTGCCTCTAAAGATCC 55.0 43.5 57.8 57.3 801-961 bp 1.0 60.1