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psbT-psbN

LSC · 74188-74249 · 62 bp

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Pi 0.0449

MarkerSeek score 71.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 8
pi: 0.0449
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	CCTTCGAAGAACATGGGGAC	47.6	55.0	57.6	58.3	124 bp	1.0	72.5
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	CCCTAGTCGCCATCTCCATA	47.6	55.0	57.6	58.1	220 bp	1.0	71.5
psbT-psbN_p3	TCGAGAACCACCTAAAGTTCC	CCTTCGAAGAACATGGGGAC	47.6	55.0	57.3	58.3	125 bp	1.0	70.3
psbT-psbN_p4	TCGAGAACCACCTAAAGTTCC	CCCTAGTCGCCATCTCCATA	47.6	55.0	57.3	58.1	221 bp	1.0	69.3
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	GCCATCTCCATATCCGGTTT	47.6	50.0	57.6	57.4	212 bp	1.0	67.7