Hotspot

atpB-rbcL

LSC · 53388-54388 · 1001 bp

Back to Holcoglossum

Pi 0.0102

MarkerSeek score 68.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 42
pi: 0.0102
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	1096-1200 bp	1.0	85.5
atpB-rbcL_p2	CGCCCTTGGTTTTGTTCTTC	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.9	58.1	1042-1146 bp	1.0	85.0
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1064-1168 bp	1.0	85.0
atpB-rbcL_p4	TACGCCCTTGGTTTTGTTCT	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.6	58.1	1044-1148 bp	1.0	83.2
atpB-rbcL_p5	ATCTTGCCCGGTGGAAAAAT	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.1	58.4	1097-1201 bp	1.0	83.1