Hotspot

trnF-GAA-trnV-UAC

LSC · 49697-50372 · 676 bp

Back to Holcoglossum

Pi 0.105

MarkerSeek score 91.30

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 170
pi: 0.105
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 91.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	677-2176 bp	1.0	90.0
trnF-GAA-trnV-UAC_p2	GGAAATGGTCGGGATAGCTC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.8	57.8	723-2222 bp	1.0	89.1
trnF-GAA-trnV-UAC_p3	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	720-2219 bp	1.0	88.8
trnF-GAA-trnV-UAC_p4	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	719-2218 bp	1.0	88.8
trnF-GAA-trnV-UAC_p5	CTCTGCTAGGATGATGCACG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	743-2242 bp	1.0	88.7