Hotspot

petN-psbM

LSC · 29921-30757 · 837 bp

Back to Holcoglossum

Pi 0.011

MarkerSeek score 61.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 38
pi: 0.011
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGGCATCATTCACATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.0	58.3	938-970 bp	1.0	85.5
petN-psbM_p2	GGGGCATCATTCACATGGAT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.0	58.3	990-1022 bp	1.0	85.3
petN-psbM_p3	GGGGCATCATTCACATGGAT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.0	57.6	991-1023 bp	1.0	84.3
petN-psbM_p4	GGATAGGGGGCATCATTCAC	ACGTCAAAACAGTCAGCCAA	55.0	45.0	57.8	58.3	944-976 bp	0.923077	80.6
petN-psbM_p5	GGATAGGGGGCATCATTCAC	ATTGCTACTGCGCTGTTCAT	55.0	45.0	57.8	58.3	996-1028 bp	0.923077	80.4