Hotspot

ndhK

LSC · 51569-52336 · 768 bp

Back to Holboellia

Pi 0

MarkerSeek score 27.80

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 768
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	CCCGCCATTTGTTATTCTGC	TCGTGCTTATCCCAATTGTTG	50.0	42.9	57.8	56.9	837 bp	1.0	61.4
ndhK_p2	ACGACCCTGCATCTTTTTGA	TCGTGCTTATCCCAATTGTTG	45.0	42.9	57.7	56.9	919 bp	1.0	60.9
ndhK_p3	CCCGCCATTTGTTATTCTGC	TTCGTGCTTATCCCAATTGTTG	50.0	40.9	57.8	57.6	838 bp	1.0	58.9
ndhK_p4	ACGACCCTGCATCTTTTTGA	TTCGTGCTTATCCCAATTGTTG	45.0	40.9	57.7	57.6	920 bp	1.0	58.4
ndhK_p5	CCCGCCATTTGTTATTCTGC	CGTGCTTATCCCAATTGTTG	50.0	45.0	57.8	55.3	836 bp	1.0	57.1