Hotspot

ndhD

SSC · 118670-120199 · 1530 bp

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Pi 0.000436

MarkerSeek score 41.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 1
pi: 0.000436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGTTCTCAAAAACTCCAAACGT	TACGTTCCGGAAAACTCCAC	40.9	50.0	57.6	57.8	1749 bp	1.0	59.9
ndhD_p2	GGTTCTCAAAAACTCCAAACGT	TATTTGATTTCGAGCGCGGT	40.9	45.0	57.6	58.4	1675 bp	1.0	58.3
ndhD_p3	GGTTCTCAAAAACTCCAAACGT	CGTTCCGGAAAACTCCACTT	40.9	50.0	57.6	58.4	1747 bp	1.0	57.8
ndhD_p4	GGTTCTCAAAAACTCCAAACGT	TTATTTGATTTCGAGCGCGG	40.9	45.0	57.6	57.3	1676 bp	1.0	55.3
ndhD_p5	GGTTCTCAAAAACTCCAAACG	TACGTTCCGGAAAACTCCAC	42.9	50.0	55.9	57.8	1749 bp	1.0	54.2