Hotspot

ndhF-rpl32

SSC · 115046-116122 · 1077 bp

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Pi 0.000619

MarkerSeek score 59.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1077
variable_sites: 1
pi: 0.000619
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATTCTTTGTTGCCGTCGGAA	AAAGCTTTTACCGCTGTCCA	45.0	45.0	58.4	58.0	1276-1277 bp	1.0	76.7
ndhF-rpl32_p2	ATTCTTTGTTGCCGTCGGAA	AAGCTTTTACCGCTGTCCAA	45.0	45.0	58.4	58.0	1275-1276 bp	1.0	76.7
ndhF-rpl32_p3	AACCGGAAGTGGAACGAAAG	AAAGCTTTTACCGCTGTCCA	50.0	45.0	58.4	58.0	1225-1226 bp	1.0	76.7
ndhF-rpl32_p4	AACCGGAAGTGGAACGAAAG	AAGCTTTTACCGCTGTCCAA	50.0	45.0	58.4	58.0	1224-1225 bp	1.0	76.7
ndhF-rpl32_p5	GAACCGGAAGTGGAACGAAA	AAGCTTTTACCGCTGTCCAA	50.0	45.0	58.4	58.0	1225-1226 bp	1.0	76.6