Hotspot

ycf3-trnS-GGA

LSC · 46226-47144 · 919 bp

Back to Holboellia

Pi 0.000725

MarkerSeek score 59.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 919
variable_sites: 1
pi: 0.000725
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCTGCGATCTAGGCA	AACGTACGGAAAGAGAGGGA	45.5	50.0	58.1	58.1	1034-1035 bp	1.0	62.9
ycf3-trnS-GGA_p2	CCATTTATCTGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	45.5	50.0	58.1	58.1	1008-1009 bp	1.0	62.8
ycf3-trnS-GGA_p3	CCATTTATCTGCGATCTAGGCA	ACGTACGGAAAGAGAGGGAT	45.5	50.0	58.1	57.9	1033-1034 bp	1.0	62.5
ycf3-trnS-GGA_p4	CCATTTATCTGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	45.5	50.0	58.1	58.2	1029-1030 bp	1.0	62.4
ycf3-trnS-GGA_p5	CCATTTATCTGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	45.5	45.0	58.1	57.8	979-980 bp	1.0	62.1