Hotspot

atpH-atpI

LSC · 14077-14805 · 729 bp

Back to Holboellia

Pi 0.000914

MarkerSeek score 59.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 1
pi: 0.000914
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GGCAGAAACCAGTGGATTCA	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.1	58.0	774-776 bp	1.0	79.4
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.9	58.0	841-843 bp	1.0	79.2
atpH-atpI_p3	GATACCTTCTACGGCTTGGC	GCGAATCCATGGAAGGTCAT	55.0	50.0	58.1	58.0	870-872 bp	1.0	79.0
atpH-atpI_p4	CGATACCTTCTACGGCTTGG	GCGAATCCATGGAAGGTCAT	55.0	50.0	57.9	58.0	871-873 bp	1.0	79.0
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	786-788 bp	1.0	78.6