Hotspot

trnK-UUU-rps16

LSC · 4503-5464 · 962 bp

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Pi 0
MarkerSeek score 59.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
962
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTACCAACGGTATGGACGA AAAAAGGCGCTCAACCTACA 50.0 45.0 57.9 58.0 1150-1156 bp 1.0 79.2
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1085-1091 bp 1.0 78.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1084-1090 bp 1.0 78.9
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 1099-1105 bp 1.0 78.1
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 1094-1100 bp 1.0 78.0