Hotspot

trnH-GUG-psbA

LSC · 103-572 · 470 bp

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Pi 0.00142

MarkerSeek score 57.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 1
pi: 0.00142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TCACAATCCACTGCCTTGAG	GCTCCAGCGACAAATGGATA	50.0	50.0	57.8	58.0	529-530 bp	1.0	78.4
trnH-GUG-psbA_p2	TCACAATCCACTGCCTTGAG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.8	57.9	553-554 bp	1.0	78.2
trnH-GUG-psbA_p3	TCACAATCCACTGCCTTGAG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.8	57.9	556-557 bp	1.0	78.2
trnH-GUG-psbA_p4	TCACAATCCACTGCCTTGAG	TAGACCTAGCTGCTGTCGAA	50.0	50.0	57.8	57.9	549-550 bp	1.0	77.8
trnH-GUG-psbA_p5	TATATGATGGGCGAACGACG	GCTCCAGCGACAAATGGATA	50.0	50.0	57.7	58.0	574-575 bp	1.0	77.2