primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TCACAATCCACTGCCTTGAG	GCTCCAGCGACAAATGGATA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.236035	103	572	529-530	1.000000	78.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TCACAATCCACTGCCTTGAG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.8	57.9	0.0	13.4	0.255492	103	572	553-554	1.000000	78.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TCACAATCCACTGCCTTGAG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.264322	103	572	556-557	1.000000	78.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TCACAATCCACTGCCTTGAG	TAGACCTAGCTGCTGTCGAA	20	20	50.0	50.0	57.8	57.9	0.0	13.4	0.313871	103	572	549-550	1.000000	77.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TATATGATGGGCGAACGACG	GCTCCAGCGACAAATGGATA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.387759	103	572	574-575	1.000000	77.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTACCAACGGTATGGACGA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	57.9	58.0	10.6	7.6	0.151611	4503	5464	1150-1156	1.000000	79.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4503	5464	1085-1091	1.000000	78.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4503	5464	1084-1090	1.000000	78.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTAGCCGCACTTAAAAGCC	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	0.0	7.6	0.290178	4503	5464	1099-1105	1.000000	78.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4503	5464	1094-1100	1.000000	78.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6606	7309	777-785	1.000000	78.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCCACGACTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	15.3	16.9	0.223468	6606	7309	799-807	1.000000	78.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6606	7309	788-796	1.000000	77.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	6606	7309	807-815	1.000000	77.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCCACGACTGATCATGTCCT	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.1	58.2	15.3	0.0	0.318388	6606	7309	829-837	1.000000	77.5
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8632	9367	809-810	1.000000	51.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8632	9367	812-813	1.000000	50.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8632	9367	811-812	1.000000	50.5
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8632	9367	810-811	1.000000	49.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATTAGCAATCCGCCGCTTTA	CAAATTGAAAGACCCCTTAACCA	20	23	45.0	39.1	58.0	57.2	0.0	0.0	3.838033	8632	9367	849-850	1.000000	49.5
atpH-atpI_p1	atpH-atpI	1	GGCAGAAACCAGTGGATTCA	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	58.1	58.0	28.1	20.7	0.117428	14077	14805	774-776	1.000000	79.4
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	57.9	58.0	0.0	20.7	0.139407	14077	14805	841-843	1.000000	79.2
atpH-atpI_p3	atpH-atpI	3	GATACCTTCTACGGCTTGGC	GCGAATCCATGGAAGGTCAT	20	20	55.0	50.0	58.1	58.0	0.0	20.7	0.159962	14077	14805	870-872	1.000000	79.0
atpH-atpI_p4	atpH-atpI	4	CGATACCTTCTACGGCTTGG	GCGAATCCATGGAAGGTCAT	20	20	55.0	50.0	57.9	58.0	0.0	20.7	0.162792	14077	14805	871-873	1.000000	79.0
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.2	58.0	1.4	20.7	0.211053	14077	14805	786-788	1.000000	78.6
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATCTGCGATCTAGGCA	AACGTACGGAAAGAGAGGGA	22	20	45.5	50.0	58.1	58.1	0.0	21.1	2.154524	46226	47144	1034-1035	1.000000	62.9
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATCTGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	22	20	45.5	50.0	58.1	58.1	0.0	0.0	2.164027	46226	47144	1008-1009	1.000000	62.8
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCATTTATCTGCGATCTAGGCA	ACGTACGGAAAGAGAGGGAT	22	20	45.5	50.0	58.1	57.9	0.0	21.1	2.210503	46226	47144	1033-1034	1.000000	62.5
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CCATTTATCTGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	22	20	45.5	50.0	58.1	58.2	0.0	0.0	2.221013	46226	47144	1029-1030	1.000000	62.4
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CCATTTATCTGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	22	20	45.5	45.0	58.1	57.8	0.0	5.6	2.262328	46226	47144	979-980	1.000000	62.1
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TTCCGGGTCTAGGCCTTTTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	12.6	0.0	0.177289	48136	48514	528-530	1.000000	78.9
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	AGATCACTTCCGGGTCTAGG	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.9	57.9	0.0	0.0	0.207469	48136	48514	535-537	1.000000	78.6
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TTCCGGGTCTAGGCCTTTTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.0	57.8	12.6	0.0	0.285042	48136	48514	496-498	1.000000	78.0
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	AGATCACTTCCGGGTCTAGG	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.315222	48136	48514	503-505	1.000000	77.8
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTCCGGGTCTAGGCCTTTTA	TGTTATGTGAGCCCGCTTAG	20	20	50.0	50.0	58.0	57.7	12.6	0.0	0.354077	48136	48514	548-550	1.000000	77.5
ndhK_p1	ndhK	1	CCCGCCATTTGTTATTCTGC	TCGTGCTTATCCCAATTGTTG	20	21	50.0	42.9	57.8	56.9	0.0	4.4	2.329125	51569	52336	837-837	1.000000	61.4
ndhK_p2	ndhK	2	ACGACCCTGCATCTTTTTGA	TCGTGCTTATCCCAATTGTTG	20	21	45.0	42.9	57.7	56.9	0.0	4.4	2.391151	51569	52336	919-919	1.000000	60.9
ndhK_p3	ndhK	3	CCCGCCATTTGTTATTCTGC	TTCGTGCTTATCCCAATTGTTG	20	22	50.0	40.9	57.8	57.6	0.0	4.4	2.641512	51569	52336	838-838	1.000000	58.9
ndhK_p4	ndhK	4	ACGACCCTGCATCTTTTTGA	TTCGTGCTTATCCCAATTGTTG	20	22	45.0	40.9	57.7	57.6	0.0	4.4	2.703538	51569	52336	920-920	1.000000	58.4
ndhK_p5	ndhK	5	CCCGCCATTTGTTATTCTGC	CGTGCTTATCCCAATTGTTG	20	20	50.0	45.0	57.8	55.3	0.0	4.4	2.866155	51569	52336	836-836	1.000000	57.1
atpB-rbcL_p1	atpB-rbcL	1	GGTGGAAAGGCTACATCCAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.0	58.0	14.7	0.0	1.061388	56111	56870	919-920	1.000000	71.6
atpB-rbcL_p2	atpB-rbcL	2	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.0	58.0	9.5	0.0	1.063706	56111	56870	906-907	1.000000	71.6
atpB-rbcL_p3	atpB-rbcL	3	GGTGGAAAGGCTACATCCAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.0	58.1	14.7	0.0	1.094675	56111	56870	918-919	1.000000	71.3
atpB-rbcL_p4	atpB-rbcL	4	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.0	58.1	9.5	0.0	1.096994	56111	56870	905-906	1.000000	71.3
atpB-rbcL_p5	atpB-rbcL	5	TTTGAGCAATACGACCCAGG	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.125215	56111	56870	885-886	1.000000	71.1
rps3_p1	rps3	1	CCATTGCTGGATAGAACGGA	ACTGAAATGGGCTACTAGCG	20	20	50.0	50.0	57.4	57.4	0.0	1.9	1.224297	85097	85753	724-724	1.000000	70.3
rps3_p2	rps3	2	TCCATTGCTGGATAGAACGG	ACTGAAATGGGCTACTAGCG	20	20	50.0	50.0	57.4	57.4	0.0	1.9	1.224297	85097	85753	725-725	1.000000	70.3
rps3_p3	rps3	3	CCATTGCTGGATAGAACGGA	ATCGGGACAGACTGAAATGG	20	20	50.0	50.0	57.4	57.3	0.0	10.1	1.319106	85097	85753	734-734	1.000000	69.6
rps3_p4	rps3	4	TCCATTGCTGGATAGAACGG	ATCGGGACAGACTGAAATGG	20	20	50.0	50.0	57.4	57.3	0.0	10.1	1.319106	85097	85753	735-735	1.000000	69.6
rps3_p5	rps3	5	CCATTGCTGGATAGAACGGA	TGAAATGGGCTACTAGCGAG	20	20	50.0	50.0	57.4	57.1	0.0	1.9	1.511059	85097	85753	722-722	1.000000	68.0
ndhF-rpl32_p1	ndhF-rpl32	1	ATTCTTTGTTGCCGTCGGAA	AAAGCTTTTACCGCTGTCCA	20	20	45.0	45.0	58.4	58.0	17.5	5.7	0.424317	115046	116122	1276-1277	1.000000	76.7
ndhF-rpl32_p2	ndhF-rpl32	2	ATTCTTTGTTGCCGTCGGAA	AAGCTTTTACCGCTGTCCAA	20	20	45.0	45.0	58.4	58.0	17.5	0.0	0.424317	115046	116122	1275-1276	1.000000	76.7
ndhF-rpl32_p3	ndhF-rpl32	3	AACCGGAAGTGGAACGAAAG	AAAGCTTTTACCGCTGTCCA	20	20	50.0	45.0	58.4	58.0	9.5	5.7	0.434538	115046	116122	1225-1226	1.000000	76.7
ndhF-rpl32_p4	ndhF-rpl32	4	AACCGGAAGTGGAACGAAAG	AAGCTTTTACCGCTGTCCAA	20	20	50.0	45.0	58.4	58.0	9.5	0.0	0.434538	115046	116122	1224-1225	1.000000	76.7
ndhF-rpl32_p5	ndhF-rpl32	5	GAACCGGAAGTGGAACGAAA	AAGCTTTTACCGCTGTCCAA	20	20	50.0	45.0	58.4	58.0	9.5	0.0	0.439043	115046	116122	1225-1226	1.000000	76.6
ccsA_p1	ccsA	1	CCCTCCTCTTTGTGCGTTTA	TCTCAAAAACTCACACACGC	20	20	50.0	45.0	57.8	56.9	0.0	0.0	1.284840	117481	118452	1075-1075	1.000000	69.7
ccsA_p2	ccsA	2	CCCTCCTCTTTGTGCGTTTA	CTCAAAAACTCACACACGCT	20	20	50.0	45.0	57.8	56.9	0.0	0.0	1.293137	117481	118452	1074-1074	1.000000	69.7
ccsA_p3	ccsA	3	CCCTCCTCTTTGTGCGTTTA	TCTCAAAAACTCACACACGCT	20	21	50.0	42.9	57.8	58.4	0.0	0.0	1.550967	117481	118452	1075-1075	1.000000	67.6
ccsA_p4	ccsA	4	CCCTCCTCTTTGTGCGTTTA	TTCTCAAAAACTCACACACGC	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.577866	117481	118452	1076-1076	1.000000	67.4
ccsA_p5	ccsA	5	CCCTCCTCTTTGTGCGTTTA	CTCAAAAACTCACACACGCTT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.584082	117481	118452	1074-1074	1.000000	67.3
ccsA-ndhD_p1	ccsA-ndhD	1	GCAATTGTGGCTTCTATCGG	AGTTGACAAGGTGGAGGCTA	20	20	50.0	50.0	57.2	58.3	7.4	0.0	1.033663	118453	118669	374-374	1.000000	72.8
ccsA-ndhD_p2	ccsA-ndhD	2	GCAATTGTGGCTTCTATCGG	GTTGACAAGGTGGAGGCTAT	20	20	50.0	50.0	57.2	57.2	7.4	0.0	1.552658	118453	118669	373-373	1.000000	68.7
ccsA-ndhD_p3	ccsA-ndhD	3	CTGCAATTGTGGCTTCTATCG	AGTTGACAAGGTGGAGGCTA	21	20	47.6	50.0	57.7	58.3	7.4	0.0	1.600157	118453	118669	376-376	1.000000	68.3
ccsA-ndhD_p4	ccsA-ndhD	4	GCAATTGTGGCTTCTATCGG	ATCAGTTGACAAGGTGGAGG	20	20	50.0	50.0	57.2	57.1	7.4	0.0	1.630358	118453	118669	377-377	1.000000	68.0
ccsA-ndhD_p5	ccsA-ndhD	5	TGCAATTGTGGCTTCTATCG	AGTTGACAAGGTGGAGGCTA	20	20	45.0	50.0	56.5	58.3	7.4	0.0	1.765929	118453	118669	375-375	1.000000	66.9
ndhD_p1	ndhD	1	GGTTCTCAAAAACTCCAAACGT	TACGTTCCGGAAAACTCCAC	22	20	40.9	50.0	57.6	57.8	0.0	20.8	2.519266	118670	120199	1749-1749	1.000000	59.9
ndhD_p2	ndhD	2	GGTTCTCAAAAACTCCAAACGT	TATTTGATTTCGAGCGCGGT	22	20	40.9	45.0	57.6	58.4	0.0	12.2	2.718554	118670	120199	1675-1675	1.000000	58.3
ndhD_p3	ndhD	3	GGTTCTCAAAAACTCCAAACGT	CGTTCCGGAAAACTCCACTT	22	20	40.9	50.0	57.6	58.4	0.0	20.8	2.776097	118670	120199	1747-1747	1.000000	57.8
ndhD_p4	ndhD	4	GGTTCTCAAAAACTCCAAACGT	TTATTTGATTTCGAGCGCGG	22	20	40.9	45.0	57.6	57.3	0.0	10.7	3.096083	118670	120199	1676-1676	1.000000	55.3
ndhD_p5	ndhD	5	GGTTCTCAAAAACTCCAAACG	TACGTTCCGGAAAACTCCAC	21	20	42.9	50.0	55.9	57.8	0.0	20.8	3.229123	118670	120199	1749-1749	1.000000	54.2
ycf1_p1	ycf1	1	AAAATCTCCTTGGCCTGCTT	AAGATTCAACCGTGGCTCAT	20	20	45.0	45.0	57.7	57.5	3.3	0.0	0.812619	127995	128114	198-198	1.000000	73.9
ycf1_p2	ycf1	2	AAATCTCCTTGGCCTGCTTG	AAGATTCAACCGTGGCTCAT	20	20	50.0	45.0	58.4	57.5	3.3	0.0	0.943192	127995	128114	197-197	1.000000	72.9
ycf1_p3	ycf1	3	CAAAATCTCCTTGGCCTGCT	AAGATTCAACCGTGGCTCAT	20	20	50.0	45.0	58.4	57.5	3.3	0.0	0.943192	127995	128114	199-199	1.000000	72.9
ycf1_p4	ycf1	4	AAAATCTCCTTGGCCTGCTT	TCCCATGTCGAATCTGGAAC	20	20	45.0	50.0	57.7	57.3	3.3	10.0	1.007129	127995	128114	259-259	1.000000	72.3
ycf1_p5	ycf1	5	AAAATCTCCTTGGCCTGCTT	CCCATGTCGAATCTGGAACT	20	20	45.0	50.0	57.7	57.3	3.3	10.0	1.014575	127995	128114	258-258	1.000000	72.2
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	141590	143494	2062-2062	1.000000	77.0
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	141590	143494	2001-2001	1.000000	76.7
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	141590	143494	2005-2005	1.000000	76.6
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	141590	143494	2000-2000	1.000000	76.6
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	141590	143494	2003-2003	1.000000	75.7
ycf2_p1	ycf2	1	CTACCACCCGGTCAATTCTG	GTACTTCGAGCTTGGAACGA	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.367085	148687	149686	1129-1129	1.000000	77.1
ycf2_p2	ycf2	2	TCTACCACCCGGTCAATTCT	GTACTTCGAGCTTGGAACGA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.395925	148687	149686	1130-1130	1.000000	76.8
ycf2_p3	ycf2	3	GTTTCCACGACTCTACCACC	GTACTTCGAGCTTGGAACGA	20	20	55.0	50.0	57.9	57.7	0.0	0.0	0.418889	148687	149686	1141-1141	1.000000	76.6
ycf2_p4	ycf2	4	CTACCACCCGGTCAATTCTG	TGGTACTTCGAGCTTGGAAC	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.474785	148687	149686	1131-1131	1.000000	76.2
ycf2_p5	ycf2	5	AATCCCTCTTTCATGGCCAC	GTACTTCGAGCTTGGAACGA	20	20	50.0	50.0	57.9	57.7	8.2	0.0	0.492883	148687	149686	1101-1101	1.000000	76.1