Hotspot

ycf1

SSC · 110124-115613 · 5490 bp

Back to Hodgsonia

Pi 0.331

MarkerSeek score 74.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5490
variable_sites: 1515
pi: 0.331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGGTATTCCCAACAATCGG	TTTCGGGGAGAACCAAAAGG	50.0	50.0	58.0	58.0	732 bp	0.5	59.7
ycf1_p2	ACGGTATTCCCAACAATCGG	AGTTGTTTTCGGGGAGAACC	50.0	50.0	58.0	58.0	738 bp	0.5	59.6
ycf1_p3	ACGGTATTCCCAACAATCGG	AAATCGTCGGACCATTCAGG	50.0	50.0	58.0	58.0	474 bp	0.5	59.5
ycf1_p4	ACGGTATTCCCAACAATCGG	CGTCGGACCATTCAGGAAAT	50.0	50.0	58.0	58.0	470 bp	0.5	59.5
ycf1_p5	ACGGTATTCCCAACAATCGG	AGCTTCCATTCCCCAAACTG	50.0	50.0	58.0	58.1	706 bp	0.5	59.2