Hotspot

ndhG

SSC · 120586-121116 · 531 bp

Back to Hodgsonia

Pi 0.453

MarkerSeek score 80.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 213
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CTCCCCGTTGTTGATTGGAA	ACAATAGCTGGGCCAATAGC	50.0	50.0	58.1	58.0	1638 bp	0.5	59.1
ndhG_p2	CTCCCCGTTGTTGATTGGAA	AGGGGAATGTGTGCGATAAC	50.0	50.0	58.1	58.0	1230 bp	0.5	58.9
ndhG_p3	ATCTCCCCGTTGTTGATTGG	ACAATAGCTGGGCCAATAGC	50.0	50.0	57.9	58.0	1640 bp	0.5	58.9
ndhG_p4	ATCTCCCCGTTGTTGATTGG	AGGGGAATGTGTGCGATAAC	50.0	50.0	57.9	58.0	1232 bp	0.5	58.7
ndhG_p5	CTCCCCGTTGTTGATTGGAA	GGGGAATGTGTGCGATAACA	50.0	50.0	58.1	58.3	1229 bp	0.5	57.2