Hotspot

ndhE

SSC · 121349-121651 · 303 bp

Back to Hodgsonia

Pi 0.449

MarkerSeek score 76.00

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 122
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TTCGTTGGGACTGGTTCTTG	ACCCATGCTTCGAGTTGTTT	50.0	45.0	58.0	58.0	1441 bp	0.5	59.5
ndhE_p2	GTTATCGCACACATTCCCCT	ACCCATGCTTCGAGTTGTTT	50.0	45.0	58.0	58.0	977 bp	0.5	59.5
ndhE_p3	TTCGTTGGGACTGGTTCTTG	GCTTCGAGTTGTTTCATGCC	50.0	50.0	58.0	58.0	1435 bp	0.5	59.4
ndhE_p4	GTTATCGCACACATTCCCCT	GCTTCGAGTTGTTTCATGCC	50.0	50.0	58.0	58.0	971 bp	0.5	59.4
ndhE_p5	TTCGTTGGGACTGGTTCTTG	ACCAACACAGTCTTCCGTTC	50.0	50.0	58.0	58.1	1357 bp	0.5	59.3